Generator

Part:BBa_K3962347

Designed by: Siheng Li   Group: iGEM21_Leiden   (2021-10-12)


J23113 promoter regulated constant expression of toxin CcdB

This construct is composed of constitutive promoter J23113 (from the Anderson promoter collection) and toxin CcdB. CcdB/CcdA is a type II toxin-antitoxin system originating from the E. coli chromosome. In our project, we used this construct to create a balanced CcdA/CcdB toxin-antitoxin system for preventing horizontal gene transfer.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 177


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Categories
Parameters
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